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| Catalog_no | BDAB0038 |
| Product_name | Recombinant SARS-CoV-2(2019-nCoV) S +E+M protein |
| Category | 冠状病毒产品 |
| Applications | Immunogen |
| Size | 50ug/100ug/1mg |
| Price | 1800/2800/8800 |
| Specificity | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) |
| Formulation | Supplied as solution form in PBS, pH7.5 or lyophilized from PBS, pH7.5 |
| Source | E.coli |
| Storage_stability | Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt. |
| othername | Spike glycoprotein;Envelope small membrane protein, E protein;Membrane protein |
| Background | Protein?S?(PROS1)?is?glycoprotein?and?expressed?in?many?cell?types?supporting?its?reported?involvement?in?multiple?biological?processes?that?include?coagulation,?apoptosis,?cancer?development?and?progression,?and?the?innate?immune?response.?Known?receptors?bind?S1?are?ACE2,?angiotensin-converting?enzyme?2,?DPP4,?CEACAM?etc..?The?spike?(S)?glycoprotein?of?coronaviruses?is?known?to?be?essential?in?the?binding?of?the?virus?to?the?host?cell?at?the?advent?of?the?infection?process.?Most?notable?is?severe?acute?respiratory?syndrome?(SARS).?The?severe?acute?respiratory?syndrome-coronavirus?(SARS-CoV)?spike?(S)?glycoprotein?alone?can?mediate?the?membrane?fusion?required?for?virus?entry?and?cell?fusion.?It?is?also?a?major?immunogen?and?a?target?for?entry?inhibitors.?It's?been?reported?that?2019-nCoV?can?infect?the?human?respiratory?epithelial?cells?through?interaction?with?the?human?ACE2?receptor.?The?spike?protein?is?a?large?type?I?transmembrane?protein?containing?two?subunits,?S1?and?S2.?S1?mainly?contains?a?receptor?binding?domain?(RBD),?which?is?responsible?for?recognizing?the?cell?surface?receptor.?S2?contains?basic?elements?needed?for?the?membrane?fusion.The?S?protein?plays?key?parts?in?the?induction?of?neutralizing-antibody?and?T-cell?responses,?as?well?as?protective?immunity.Coronavirus?envelope?(E)?proteins?are?short?(100?residues)?polypeptides?that?contain?at?least?one?transmembrane?(TM)?domain?and?a?cluster?of?2-3?juxtamembrane?cysteines.?These?proteins?are?involved?in?viral?morphogenesis?and?tropism,?and?their?absence?leads?in?some?cases?to?aberrant?virions,?or?to?viral?attenuation.?In?common?to?other?viroporins,?coronavirus?envelope?proteins?increase?membrane?permeability?to?ions,?plays?a?central?role?in?virus?morphogenesis?and?assembly.?Acts?as?a?viroporin?and?self-assembles?in?host?membranes?forming?pentameric?protein-lipid?pores?that?allow?ion?transport.?Also?plays?a?role?in?the?induction?of?apoptosis.?Activates?the?host?NLRP3?inflammasome,?leading?to?IL-1beta?overproduction.Component?of?the?viral?envelope?that?plays?a?central?role?in?virus?morphogenesis?and?assembly?via?its?interactions?with?other?viral?proteins. |
| Notes | S+E+M |
| description | Recombinant SARS-CoV-2 S&M&E fusion is produced by E.coli expression system and the target gene encoding Arg319-Phe541&Arg38-Val75&Arg101-Gln222 is expressed with C-His Tag |
| Type | N-His |
| Purity | >90% as determined by SDS-PAGE |
| Molecular Weight | 44.7kDa |
| Instructions | 
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